James Hutchins
Publications
Articles on which I am an author. * indicates equal contributions.
> Access publications in PDF format (Google Drive folder)
2020
Hutchins JRA (2020) “Genomic Databases” in Genome Plasticity in Health and Disease. D. Forero & G. Patrinos, Eds. Elsevier Press. Pages 47-62.
Abstract (publisher's site)
2018
Ivanov MP, Ladurner R, Poser I, Beveridge R, Rampler E, Hudecz O, Novatchkova M, Hériché JK, Wutz G, van der Lelij P, Kreidl E, Hutchins JRA, Axelsson‐Ekker H, Ellenberg J, Hyman AA, Mechtler K, Peters JM (2018) "The replicative helicase MCM recruits cohesin acetyltransferase ESCO2 to mediate centromeric sister chromatid cohesion" The EMBO Journal 37, e97150. PubMed • Journal site
2017
Hutchins JRA (2017) “Interrogating the genome: approaches and tools for accessing genomic databases” Atlas of Science (lay summary). Full article (HTML) • PDF
Hutchins JRA (2017) “Genomic Database Searching” in Bioinformatics, Volume I: Data, Sequence Analysis, and Evolution (Keith JM, Ed.) Methods Mol Biol. 1525, 225-269. Abstract (PubMed) • Publisher's website • Access article
2016
Hutchins JRA*, Aze A*, Coulombe P*, Méchali M (2016) “Characteristics of Metazoan DNA Replication Origins”, from DNA Replication, Recombination, and Repair (Hanaoka F & Sugasawa K, Eds.), Chapter 2, pp 23-52. Abstract • Access Article
Hutchins JRA, Traver S, Coulombe P, Peiffer I, Kitzmann M, Latreille D, Méchali M (2016) “Proteomic data on the nuclear interactome of human MCM9” Data In Brief 6, 410-415. PubMed • Full article online with link to Download PDF
Korthout T*, Emanuelli G*, Hutchins JRA (2016) “Extracting order from heterogeneity: A report on the EpiGeneSys workshop 'Single Cell Epigenetics' in Montpellier, June 11-12, 2015” BioEssays 38(1):4-7. PubMed • Access article PDF
2015
Traver S*, Coulombe P*, Peiffer I, Hutchins JRA, Kitzmann M, Latreille D, Méchali M (2015) “MCM9 Is Required for Mammalian DNA Mismatch Repair” Molecular Cell 59(5):831-9. PubMed • Full-text HTML • PDF • Mass spec data in PRIDE database
2014
Hutchins, JRA (2014) “What’s that gene (or protein)? Online resources for exploring functions of genes, transcripts, and proteins” Mol. Biol. Cell 25:8 1187-1201; doi:10.1091/mbc.E13-10-0602. Invited Technical Perspective article.
Main article: PDF • Full-text HTML • PMC Classic • PubReader • ePub
Supplementary Materials PDF: Updated Supplementary Materials v142c
• Recommended three times by Faculty of 1000 (F1000Prime)
2012
Barbet R*, Peiffer I*, Hutchins JRA, Hatzfeld A, Garrido E, Hatzfeld JA (2012) “Expression of the 49 human ATP binding cassette (ABC) genes in pluripotent embryonic stem cells and in early- and late-stage multipotent mesenchymal stem cells: Possible role of ABC plasma membrane transporters in maintaining human stem cell pluripotency” Cell Cycle 11 1610-9. Abstract • PDFs: Main article • Supplement
2011
Hegemann B*, Hutchins JRA*, Hudecz O, Novatchkova M, Rameseder J, Sykora MM, Liu S, Mazanek M, Lénárt P, Hériché J-K, Poser I, Kraut N, Hyman AA, Yaffe MB, Mechtler K, Peters JM (2011) “Systematic phosphorylation analysis of human mitotic protein complexes” Science Signaling 4 (198) rs12. Abstract • Editor's Summary
Alexander J, Lim D, Joughin BA, Hegemann B, Hutchins JRA, Ehrenberger T, Ivins F, Sessa F, Hudecz O, Nigg EA, Fry AM, Musacchio A, Stukenberg PT, Mechtler K, Peters JM, Smerdon SJ, Yaffe MB. (2011) “Spatial exclusivity combined with positive and negative selection of phosphorylation motifs is the basis for context-dependent mitotic signaling” Science Signaling 4 (179) ra42. Abstract • Editor's Summary
2010
Hutchins JRA*, Toyoda Y*, Hegemann B*, Poser I*, Hériché JK, Sykora MM, Augsburg M, Hudecz O, Buschhorn BA, Bulkescher J, Conrad C, Comartin D, Schleiffer A, Sarov M, Pozniakovsky A, Slabicki MM, Schloissnig S, Steinmacher I, Leuschner M, Ssykor A, Lawo S, Pelletier L, Stark H, Nasmyth K, Ellenberg J, Durbin R, Buchholz F, Mechtler K, Hyman AA, Peters JM (2010) “Systematic analysis of human protein complexes identifies chromosome segregation proteins” Science 328 593-9. Abstract • Full Text (PubMed Central)
• Reviewed in Rusk, N. (2010) "Discovering Mozart" Nature Methods 7 p.421
Schmitz MHA, Held M, Janssens V, Hutchins JRA, Hudecz O, Ivanova E, Goris J, Trinkle-Mulcahy L, Lamond AI, Poser I, Hyman AA, Mechtler K, Peters JM, Gerlich DW (2010) “Live-cell imaging RNAi screen identifies PP2A–B55α and importin-β1 as key mitotic exit regulators in human cells” Nature Cell Biol 12 886-93. Abstract
Dikovskaya D, Li Z, Newton IP, Davidson I, Hutchins JRA, Kalab P, Clarke PR, Näthke IS (2010) “Microtubule assembly by the Apc protein is regulated by importin-beta-RanGTP” J Cell Sci 123 736-46. Full text • PDF
Mazanek M, Roitinger E, Hudecz O, Hutchins JRA, Hegemann B, Mitulović G, Taus T, Stingl C, Peters JM, Mechtler K (2010) “A new acid mix enhances phosphopeptide enrichment on titanium- and zirconium dioxide for mapping of phosphorylation sites on protein complexes” J Chromatography B 878 515-24. Abstract
2009
Hutchins JRA, Moore WJ, Clarke PR (2009) “Dynamic localisation of Ran GTPase during the cell cycle” BMC Cell Biol 10 66. Full text • PDF
Lawo S, Bashkurov M, Mullin M, Gomez Ferreria M, Kittler R, Habermann B, Tagliaferro A, Poser I, Hutchins JRA, Hegemann B, Pinchev D, Buchholz F, Peters JM, Hyman AA, Gingras AC, Pelletier L (2009) “HAUS, the 8-Subunit Human Augmin Complex, Regulates Centrosome and Spindle Integrity” Current Biol 19 816-26. Abstract
Mitulović G, Stingl C, Steinmacher I, Hudecz O, Hutchins JRA, Peters JM, Mechtler K (2009) “Preventing carryover of peptides and proteins in nano LC-MS separations” Analytical Chem 15 (14) 5955-60. Abstract
2008
Poser I*, Sarov M*, Hutchins JRA, Hériché JK, Toyoda Y, Pozniakovsky A, Weigl D, Nitzsche A, Hegemann B, Bird A, Pelletier L, Kittler R, Hua S, Naumann R, Augsburg M, Sykora MM, Zhang Y, Nasmyth K, White KP, Dietzel S, Mechtler K, Durbin R, Stewart AF, Peters JM, Buchholz F, Hyman AA (2008) “BAC TransgeneOmics: A high-throughput method for exploration of protein function in mammals” Nature Methods 5 409-15. Abstract
2007
Jiménez JL, Hegemann B, Hutchins JRA, Peters JM, Durbin R (2007) “A systematic comparative and structural analysis of protein phosphorylation sites based on the mtcPTM database” Genome Biol 8 (5) R90. Full text • PDF
Mazanek M, Mitulovic G, Herzog F, Stingl C, Hutchins JRA, Peters JM, Mechtler K (2007) “Titanium dioxide as a chemo-affinity solid phase in offline phosphopeptide chromatography prior to HPLC-MS/MS analysis” Nature Protocols 2 1059-69. Abstract
2006
Mujezinovic N, Raidl G, Hutchins JRA, Peters JM, Mechtler K, Eisenhaber F (2006) “Cleaning of raw peptide MS/MS spectra: improved protein identification following deconvolution of multiply charged peaks, isotope clusters, and removal of background noise” Proteomics 6 (19) 5117-31. Abstract • PDF • MS Cleaner program
2004
Hutchins JRA, Clarke PR (2004) “Many Fingers on the Mitotic Trigger: Post-Translational Regulation of the Cdc25C Phosphatase” Cell Cycle 3 41-5. Abstract • PDF
Hutchins JRA, Moore WJ, Hood FE, Wilson JSJ, Andrews PD, Swedlow JR, Clarke PR (2004) “Phosphorylation regulates the dynamic interaction of RCC1 with chromosomes during mitosis” Current Biol 14 1099-104. Abstract
2003
Hutchins JRA, Dikovskaya D, Clarke PR (2003) “Regulation of Cdc2/cyclin B activation in Xenopus egg extracts via inhibitory phosphorylation of Cdc25C phosphatase by Ca2+/ calmodulin-dependent kinase II” Mol Biol Cell 14 4003-14. Full text • PDF
2002
Hutchins JRA, Dikovskaya D, Clarke PR (2002) “Dephosphorylation of the inhibitory phosphorylation site S287 in Xenopus Cdc25C by protein phosphatase-2A is inhibited by 14‑3‑3 binding” FEBS Lett 528 267-71. Abstract
Zhang C, Hutchins JRA, Mühlhäusser P, Kutay U, Clarke PR (2002) “Role of Importin‑beta in the Control of Nuclear Envelope Assembly by Ran” Current Biol 12 498-502. Abstract
2001
Curman D, Cinel B, Williams DE, Rundle N, Block WD, Goodarzi AA, Hutchins JR, Clarke PR, Zhou BB, Lees-Miller SP, Andersen RJ, Roberge M (2001) “Inhibition of the G2 DNA Damage Checkpoint and of Protein Kinases Chk1 and Chk2 by the Marine Sponge Alkaloid Debromohymenialdisine” J Biol Chem 276 17914-9. Full text • PDF
2000
Hutchins JRA, Hughes M, Clarke PR (2000) “Substrate specificity determinants of the checkpoint protein kinase Chk1” FEBS Lett 466 91-5. Abstract
Luciani MG, Hutchins JRA, Zheleva D, Hupp TR (2000) “The C-terminal Regulatory Domain of p53 Contains a Functional Docking Site for Cyclin A” J Mol Biol 300 503-18. Abstract • View first page
PATENT
Hegemann B, Peters JM, Hudecz O, Hutchins JRA, Kueng S, Mechtler K (2008) "Biomarkers for Polo-like Kinase Acitivity" European Patent EP 1884523
